重组马白介素-1受体拮抗剂蛋白蛋白 rEqIL-1RA

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产品价格： < 面议

产品型号：rEqIL-1RA

品牌：KALANG

公司名称：上海康朗生物科技有限公司

所在地：上海

浏览次数：1438 次

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产品简介

重组马白介素-1受体拮抗剂蛋白蛋白与其它公司提供的重组蛋白不同，rEqIL-1RA蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组马白介素-1受体拮抗剂蛋白蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品详细信息

重组马白介素-1受体拮抗剂蛋白蛋白

Synonyms	IL-1RN, IRAP
Species	Equine
Accession	O18999
GeneID	100034236
Source	重组马白介素-1受体拮抗剂蛋白蛋白Escherichia coli.
Molecular Weight	Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Quantity	5µg/20µg/1000µg
AA Sequence	重组马白介素-1受体拮抗剂蛋白蛋白HPLGKRPCKM QAFRIWDVNQ KTFYMRNNQL VAGYLQESNT KLQEKIDVVP IEPDALFLGL HGRKLCLACV KSGDEIRFQL EAVNITDLSK NKEENKRFTF IRSNSGPTTS FESAACPGWF LCTAQEADRP VSLTNKPKES FMVTKFYLQE DQ
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 3.0 μg/ml, corresponding to a specific activity of > 333 IU/mg in the presence of 50 pg/ml rHuIL-1α.
Physical Appearance	重组马白介素-1受体拮抗剂蛋白蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/µg of rEqIL-1RA as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组马白介素-1受体拮抗剂蛋白蛋白
SDS-PAGE
Reference	1. Dinarello CA. 1994. FASEB J. 8:1314-25. 2. Patterson D, Jones C, Hart I, et al. 1993. Genomics. 15:173-6. 3. Steinkasserer A, Spurr NK, Cox S, et al. 1992. Genomics. 13:654-7. 4. Perrier S, Darakhshan F, Hajduch E. 2006. FEBS Lett. 580:6289-94. 5. Gabay C, Porter B, Fantuzzi G, et al. 1997. J Immunol. 159:5905-13.
Background	IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. It is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-1RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The equus caballus IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26 % amino acid homology to IL-1β and 19 % homology to IL-1α. It also shares 78 %~80 % a.a. sequence identity with murine, rat, porcine IL-1RA.