钙激活的中性蛋白酶2抗体 Calpain 2

钙激活的中性蛋白酶2抗体

规格：1mg/1ml

英文名： Calpain 2

别名： Calcium activated neutral proteinase 2; Calcium activated neutral proteinase; CALP80; Calpain 2 (m/II) large subunit; Calpain 2 catalytic subunit; Calpain 2 large catalytic subunit; Calpain 2 large su

分子量： 78kDa

储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce

克隆类型：Polyclonal

亚型：IgG

纯化方法：affinity purified by Protein A

**原：KLH conjugated synthetic peptide derived from mouse Calpain

交叉反应：Human, Mouse, Rat, Dog, Cow, Sheep,

细胞定位：细胞浆 细胞膜

钙激活的中性蛋白酶2抗体产品介绍：background: Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The钙激活的中性蛋白酶2抗体 calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux. Function: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (By similarity). Subunit: Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Subcellular Location: Cytoplasm (By similarity). 钙激活的中性蛋白酶2抗体Cell membrane (By similarity). Note=Translocates to the plasma membrane upon Ca(2+) binding (By similarity). Tissue Specificity: Ubiquitous. Similarity: Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 2 EF-hand domains. Gene ID: 12334 Database links: Entrez Gene: 824 Human Entrez Gene: 12334 Mouse Entrez Gene: 29154 Rat Omim: 114230 Human SwissProt: P17655 Human SwissProt: O08529 Mouse SwissProt: Q07009 Rat Unigene: 350899 Human Unigene: 19306 Mouse Unigene: 6822 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 钙蛋白酶2(calpain2)也是一族钙离子依赖性的、水解半胱氨酸的蛋白酶,广泛存在于全身各脏器组织中。钙蛋白酶参与不同细胞过程,包括细胞骨架和细胞膜附着的构型重建、不同的信号转导途径和细胞凋亡等.

钙激活的中性蛋白酶2抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

研究领域：肿瘤  细胞生物  信号转导  新陈代谢  

储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

来源： Rabbit

外观： Lyophilized or Liquid