MSU诱导的腹膜炎模型
产品简介
实验方案: 1. MSU用PBS重悬, 每只实验鼠腹腔注射1mgMSU; 2. 6小时后取腹腔灌洗液, 用10mL PBS冲洗; 3. 分析灌洗液中中性粒细胞数量,流式细胞检测,细胞表面分子: Ly-6G和CD11b
产品详细信息
实验方案:
1. MSU用PBS重悬, 每只实验鼠腹腔注射1mgMSU;
2. 6小时后取腹腔灌洗液, 用10mL PBS冲洗;
3. 分析灌洗液中中性粒细胞数量,流式细胞检测,细胞表面分子: Ly-6G和CD11b
Brief protocol of MSU induced mouse peritonitis model
1. Mice were i.p. injected of 1mg MSU crystals in 0.5 ml PBS;
2. 6h later, animals were killed by CO2 exposure;
3. Peritoneal cavities were washed with 10 ml of PBS;
4. The lavage fluids were analyzed for neutrophil recruitment by fluorescence-activated cell sorting using the neutrophil markers Ly-6G and CD11b。