订购货号 |
产品名称及规格 |
价格(¥) |
11020 |
Cyanine3 NHS ester, 1 mg |
1430.00 |
21020 |
Cyanine3 NHS ester, 5 mg |
2730.00 |
41020 |
Cyanine3 NHS ester, 25 mg |
5330.00 |
51020 |
Cyanine3 NHS ester, 50 mg |
9035.00 |
61020 |
Cyanine3 NHS ester, 100 mg |
15470.00 |
Cyanine3 NHS ester是标记生物分子的氨基基团的活性染料,是Cy3® NHS ester的类似物。该荧光染料是可溶性蛋白、多肽和寡核苷酸/DNA的理想标记物。对于痕量蛋白质,可以考虑用水溶的不需要共溶剂的sulfo-Cyanine3 NHS ester。
Cyanine3 NHS ester可以替代Cy3®, Alexa Fluor 546, and DyLight 549。
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Cy® is a trademark of GE Healthcare.
General properties
Appearance:
|
red powder
|
Mass spec M+ increment:
|
474.2
|
Molecular weight:
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590.15
|
Molecular formula:
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C34H40ClN3O4
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Solubility:
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soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
|
Quality control:
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NMR 1H and HPLC-MS (95%)
|
Storage conditions:
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Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
|
MSDS:
|
Download
|
Spectral properties
Excitation maximum, nm:
|
555
|
Extinction coefficient at excitation maximum, Lmol-1cm-1:
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150000
|
Emission maximum, nm:
|
570
|
Fluorescence quantum yield:
|
0.31
|
Product citations
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Cao, Z.; Partyka, K.; McDonald, M.; Brouhard, E.; Hincapie, M.; Brand, R.E.; Hancock, W.S.; Haab, B.B. Modulation of Glycan Detection on Specific Glycoproteins by Lectin Multimerization. Analytical Chemistry, 2013, 85(3), 1689-1698. doi: 10.1021/ac302826a
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Haller, A.; Altman, R.B.; Souliere, M.F.; Blanchard, S.C.; Micura, R. Folding and ligand recognition of the TPP riboswitch aptamer at single-molecule resolution. Proceedings of the National Academy of Sciences, 2013, 110(11), 4188-4193. doi: 10.1073/pnas.1218062110
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Kaastrup, K.; Chan, L.; Sikes, H.D. Impact of Dissociation Constant on the Detection Sensitivity of Polymerization-Based Signal Amplification Reactions. Analytical Chemistry, 2013, 85(17), 8055-8060. doi: 10.1021/ac4018988
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Gatzogiannis, E.; Chen, Z.; Wei, L.; Wombacher, R.; Kao, Y.-T.; Yefremov, G.; Cornish, V.W.; Min, W. Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag. Chemical Communications, 2012, 48(69), 8694-8694. doi: 10.1039/c2cc33133k
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Kaastrup, K.; Sikes, H.D. Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times. Lab on a Chip, 2012, 12(20), 4055-4055. doi: 10.1039/c2lc40584a
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Choi, E.B.; Choi, J.; Bae, S.R.; Kim, H.-O.; Jang, E.; Kang, B.; Kim, M.-H.; Kim, B.; Suh, J.-S.; Lee, K. et al. Colourimetric redox-polyaniline nanoindicator for in situ vesicular trafficking of intracellular transport. Nano Research, 2014. doi: 10.1007/s12274-014-0597-6
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Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
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Kim, J.; Yang, Y.; Song, S.S.; Na, J.-H.; Oh, K.J.; Jeong, C.; Yu, Y.G.; Shin, Y.-K. Beta-Amyloid Oligomers Activate Apoptotic BAK Pore for Cytochrome c Release. Biophysical Journal, 2014, 107(7), 1601-1608. doi: 10.1016/j.bpj.2014.07.074
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Albertazzi, L.; Martinez-Veracoechea, F.J.; Leenders, C.M.A.; Voets, I.K.; Frenkel, D.; Meijer, E.W. Spatiotemporal control and superselectivity in supramolecular polymers using multivalency. Proceedings of the National Academy of Sciences, 2013, 110(30), 12203-12208. doi: 10.1073/pnas.
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