英文名称:L-AAO;TM-LAO;L-Amino Acid Oxidase
CAS号:9000-89-9
级别:BR
类型:Type IV
来源:蝮蛇毒(Crotalus adamanteus venom)
分子量:63000Da
活力:≥4u/mg
活力定义:One unit oxidizes one micromile of L-leucine per minute at 25℃,PH7.6 (L-Leucine is deaminated at the same rate at pH 7.8 at 37 ℃)
蛋白质(Biuret):≥50mg/ml
描述:Supplied as an aqueous solution with toluene added as a preservative.The presence of substrate and absence of oxygen stabilize the enzyme at elevated temperatures.The enzyme may be reversibly inactivated by incubation at 38℃ in phosphate buffer,PH7.5
性状:冻干白色粉末或悬浮液。由两个非共价的亚基组成,每个亚基的分子量为55KD,TM-LAO的N末端氨基酸序列是ADNKNPLEECFRETNYEEFLEIAR
用途:生化研究。能够抑制由ADP和胶原所引起的血小板聚集。具有细胞毒性,能诱导细胞凋亡。能抑制大肠杆菌,金黄色葡萄球菌和痢疾杆菌的生长,杀死肿瘤细胞以及诱导血小板聚集,这些活性能被过氧化氢酶所抑制。
保存:-20℃
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