SignaGen生物技术公司于2006年成立于美国马里兰州。公司的创始人系几个来自美国国立卫生研究院(National Institutes of health, NIH)的致力于生物医学研究的科学家。
自SignaGen生物技术公司建立的**天起, 他们就一直致力于成为美国基因转导及基因**领域的先驱. 目前SignaGen公司已经成功地开发出三大系列分别用于体内和体外的DNA和RNA转染试剂, 是目前世界上**拥有从多聚阳离子聚合物到阳离子型脂质体转染试剂的生物公司.
当他们还在NIH从事生物医学基础研究时, 他们就经常需要将外源性基因导入到哺乳动物细胞. 他们一方面惊讶于DNA转染试剂出色的转染效率, 另一方面他们也惊讶于转染试剂高的离谱的价格. 从那一刻起, 他们开始尝试着用老掉牙的旋转蒸发仪在自己的实验室制作基于脂质体的DNA转染试剂. 经过耗时费力的程序及制剂的优化工作, 他们自制的DNA转染试剂同市场上好的转染试剂一样出色, 但是成本只是这些商用转染试剂的零头. 从那时起, 他们开始质疑这些商用转染试剂高得离谱的价格. 他们为什么不可以自己制作和销售这些转染试剂? 他们为什么不可以为他们的同行们提供高效率, 低价位的转染试剂呢? 基于这一想法, 他们成立了该公司并开始研制开发DNA和RNA转染试剂.
2007年1月, 他们向市场投放**个DNA转染试剂------GenJet™ 体外DNA转染试剂, 该试剂因为极高的转染效率和极低的毒性迅速获得生物医学科研工作者的青睐. 接下来他们迅速地升级了该转染试剂, 先后研制出了GenJet™(版本II)和GenJet™ Plus等系列**升级产品. 这些升级产品因为显著地增加了DNA结合基团, 所以转染效率更高. 近他们又先后研发成功了基于多聚阳离子聚合物的转染试剂-------PolyJet™体外DNA转染试剂和基于脂质体的LipoD293™ 体外DNA转染试剂. PolyJet™体外DNA转染试剂一种新型的生物可降解的聚合物, 进入细胞后, 该聚合物迅速完全地降解, 从而几乎不产生细胞毒性; LipoD293™体外DNA转染试剂则为一个加强型DNA转染试剂, 因为其中添加了多肽增强剂, 所以其转染效率非常高, 适用于难以转染的哺乳动物细胞.
让我们拭目以待, 看一看接下来的几个月他们能为您带来怎样的惊喜!
SignaGen生物公司, 一个基因转导/基因**公司, 我们的口号: “Combine Efficiency & Affordability..."
美国近出版的生物专业书刊对SignaGen转染试剂的评价(google)搜索：
1. Run-Qiang Chen, Qing-Kai Yang, Yan-Ling Chen, Vasco A. Oliveira, William S. Dalton, Colleen Fearns, and Jiing-Dwan Lee. Kinome Sirna screen Identifies SMG-1 as a Negative Regulator of Hypoxia-inducible Factor-1α in Hypoxia.. J Biol Chem. 2009; 284(25): 16752–16758.
2. Thomas E. Taylor-Clark, Srinivas Ghatta, Weston Bettner, and Bradley J. Undem. Nitrooleic Acid, an Endogenous Product of Nitrative Stress, Activates Nociceptive Sensory Nerves via the Direct Activation of TRPA1. Molecular Pharmacology, 2009, to be published, available on web
3. Luke C. McSpadden, Robert D. Kirkton, and Nenad Bursac. lectrotonic loading of anisotropic cardiac monolayers by unexcitable cells depends on connexin type and expression level. Am J Physiol Cell Physiol, 2009; 297:C339-C351
4. Run-qiang Chen, Qing-kai Yang, Bing-wen Lu, Wei Yi, Greg Cantin, Yan-ling Chen, Colleen Fearns, John R. Yates, III and Jiing-Dwan Lee. CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells. Cancer Research, 2009; 69:2663
5. Peter Klover, Weiping Chen, Bing-Mei Zhu, and Lothar Hennighausen. Skeletal muscle growth and fiber composition in mice are regulated through the transcription factors STAT5a/b: linking growth hormone to the androgen receptor. FASEB Journal, 2009, to be published, available on web
6. Thomas E. Taylor-Clark, Srinivas Ghatta, Weston Bettner and Bradley J. Undem#. Nitrooleic acid, an Endogenous Product of Nitrative Stress, Activates Nociceptive Sensory Nerves via the Direct Activation of TRPA1. Molecular Pharmacology, 2009, to be published, available on web
7. Krista A. Delviks-Frankenberry, Galina N. Nikolenko, Frank Maldarelli, Saiki Hase, Yutaka Takebe, and Vinay K. Pathak. Subtype-Specific Differences in the Human Immunodeficiency Virus Type 1 Reverse Transcriptase Connection Subdomain of CRF01_AE Are Associated with Higher Levels of Resistance to 3'-Azido-3'-Deoxythymidine. Journal of Virology, September 2009, 83: 8502-8513
8. Bin Jia, Ruth Serra-Moreno, William Neidermyer, Jr., Andrew Rahmberg, John Mackey, Ismael Ben Fofana, Welkin E. Johnson, Susan Westmoreland, David T. Evans. Species-Specific Activity of SIV Nef and HIV-1 Vpu in Overcoming Restriction by Tetherin/BST2. PLoS Pathogens, 2009, to be published, available on web
9. Wen-Bin Tsai, Young Min Chung, Yoko Takahashi, Zhaohui Xu, and Mickey C-T. Hu. Functional interaction between FOXO3a and ATM regulates DNA damage response. Nat Cell Biol. 2008; 10(4): 460–467
10. Rothenburg S, Seo EJ, Gibbs JS, Dever TE, Dittmar K.. Rapid evolution of protein kinase PKR alters sensitivity to viral inhibitors. Nat Struct Mol Biol. 2009; 16(1):63-70
11. J-H Zhu, R Chen, W Yi, G T Cantin, C Fearns, Y Yang, J R Yates III and J-D Lee. Protein tyrosine phosphatase PTPN13 negatively regulates Her2/ErbB2 malignant signaling. Oncogene 2008; 27: 2525-31
12. Krista A. Delviks-Frankenberry, Galina N. Nikolenko, Paul L. Boyer, Stephen H. Hughes, John M. Coffin, Abhay Jere, and Vinay K. Pathak. HIV-1 reverse transcriptase connection subdomain mutations reduce template RNA degradation and enhance AZT excision. Proc Natl Acad Sci U S A. 2008; 105: 10943–10948
13. Jianhua Zhao, Zhenping Zhang, Zivjena Vucetic, Kenneth J. Soprano, Dianne Robert Soprano. HACE1: A novel repressor of RAR transcriptional activity. Journal of Cellular Biochemistry, 2008; 107: 482-493
14. Rebecca Klinger and Nenad Bursac. Cardiac Cell Therapy In Vitro: Reproducible Assays for Comparing the Efficacy of Different Donor Cells. IEEE Eng Med Biol Mag. Author manuscript; available in PMC 2009 July 24
15. Shiling Yang, Shouzou Wei, Ambra Pozzi and Jorge H. Capdevila. The arachidonic acid epoxygenase is a component of the signaling mechanisms responsible for VEGF-stimulated angiogenesis. Archives of Biochemistry and Biophysics, 2009 to be published, available on web
16. SALLY DABELSTEEN,a,b PAULA HERCULE,a PATRICIA BARRON,a MEGHAN RICE, GREGORY DORSAINVILLE, JAMES G. RHEINWALD. Epithelial Cells Derived from Human Embryonic Stem Cells Display P16INK4A Senescence, Hypermotility, and Differentiation Properties Shared by Many P631 Somatic Cell Types. STEM CELLS, 2009;27:1388–1399
17. Boris B. Boyanovsky1, Preetha Shridas2, Michael Simons, Deneys R. van der Westhuyzen1,2,3, and Nancy R. Webb. Syndecan-4 Mediates Macrophage Uptake of Group V Secretory Phospholipase A2-Modified Low Density Lipoprotein. J Lipid Research, 2009 to be published, available on web
18. Young Yil Bahk, Ick-Hyun Cho and Tong Soo Kim. A Cross-talk between oncogenic Ras and tumor suppressor PTEN through FAK Tyr861 phosphorylation in NIH/3T3 mouse embryonic fibroblasts. Biochemical and Biophysical Research Communications, 2008; 377: 1199-1204
19. Grzegorz Woszczek, Li-Yuan Chen, Sahrudaya Nagineni, Sara Alsaaty, Anya Harry, Carolea Logun, Rafal Pawliczak, and James H. Shelhamer. IFN- Induces Cysteinyl Leukotriene Receptor 2 Expression and Enhances the Responsiveness of Human Endothelial Cells to Cysteinyl Leukotrienes. The Journal of Immunology, 2007;178: 5262-5270
20. Jean-Marie Sontag,1 Viyada Nunbhakdi-Craig,1 Lisa Montgomery,1 Erland Arning,2 Teodoro Bottiglieri,2 and Estelle Sontag. Folate Deficiency Induces In Vitro and Mouse Brain Region-Specific Downregulation of Leucine Carboxyl Methyltransferase-1 and Protein Phosphatase 2A B_ Subunit Expression That Correlate with Enhanced Tau Phosphorylation. The Journal of Neuroscience, 2008; 28(45):11477–11487
21. Jacob, Abraham; Lee, Tina X.; Neff, Brian A.; Miller, Shyra; Welling, Bradley; Chang, Long-Sheng. Phosphatidylinositol 3-Kinase/AKT Pathway Activation in Human Vestibular Schwannoma. Otology & Neurotology, 2008; 29: 58-68
22. Rachel A. Karlnoski, Arnon Rosenthal, Dione Kobayashi, Jaume Pons, Jennifer Alamed, Mary Mercer, Qingyou Li, Marcia N. Gordon, Paul E. Gottschall, and David Morgan. Suppression of Amyloid Deposition Leads to Long-Term Reductions in Alzheimer's Pathologies in Tg2576 Mice. The Journal of Neuroscience, 2009, 29(15):4964-4971
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美国SignaGen公司官方网站：http://signagen.com/
大陆地区总代理： http://www.jnqsw.com/
