谷氨酸半胱氨酸连接酶(GCLM)重组蛋白
Recombinant Glutamate Cysteine Ligase, Modifier Subunit (GCLM)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
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Organism species
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Homo sapiens (Human)
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Product No.
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KL038Hu01
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Source
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Prokaryotic expression
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Host
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E.coli
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Purity
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> 95%
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UOM
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50ug
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Predicted Molecular Mass
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27.7kDa
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Concentration
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4.9
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Applications
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SDS-PAGE; WB; ELISA; IP.
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Endotoxin Level
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<1.0EU per 1µg (determined by the LAL method)
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Residues
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Ser40~Ser251 (Accession # P48507) with two N-terminal Tags, His-tag and T7-tag
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Formulation
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Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
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谷氨酸半胱氨酸连接酶(GCLM)重组蛋白SEQUENCES
The sequence of the target protein is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- S EELHDCIQKT LNEWSSQINP DLVREFPDVL ECTVSHAVEK INPDEREEMK VSAKLFIVES NSSSSTRSAV DMACSVLGVA QLDSVIIASP PIEDGVNLSL EHLQPYWEEL ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIQAH EWVPLWLLRY S
谷氨酸半胱氨酸连接酶(GCLM)重组蛋白USAGE
Reconstitute in sterile ddH2O.
谷氨酸半胱氨酸连接酶(GCLM)重组蛋白STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
谷氨酸半胱氨酸连接酶(GCLM)重组蛋白About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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