Anti-phospho-ARP3 (Ser418)抗体

Cat. Number:

Anti-phospho-ARP3 (Ser418)抗体KL-8999R

Quantity size:

0.1ml

Concentration:

1mg/ml   Buffer = 0.01M Anti-phospho-ARP3 (Ser418)抗体TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the Anti-phospho-ARP3 (Ser418)抗体uncapping existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, Anti-phospho-ARP3 (Ser418)抗体namely the WASP proteins, and initiates the formation of lamellipodia and filopodia.

Also known as:

ARP3 (phospho-Ser418); ARP3 (phospho S418); Actin-like protein 3; Actin-related protein 3; ACTR3; ARP3 Anti-phospho-ARP3 (Ser418)抗体actin-related protein 3 homolog; ARP3_HUMAN.

Specificity:

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500   
Not yet tested in other applications. 
Optimal working Anti-phospho-ARP3 (Ser418)抗体dilutions must be determined by the end user.