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- 产品名称：重组人胰岛素样生长因子- 1蛋白
- 产品型号：rHuIGF-1
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-20
- 在线询价
简单介绍
重组人胰岛素样生长因子- 1蛋白与其它公司提供的重组蛋白不同，rHuIGF-1蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人胰岛素样生长因子- 1蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组人胰岛素样生长因子- 1蛋白
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Synonyms
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Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF
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Species
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Human
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Accession
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重组人胰岛素样生长因子- 1蛋白P05019
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GeneID
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3479
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
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Quantity
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100µg/500µg/1000µg
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AA Sequence
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重组人胰岛素样生长因子- 1蛋白GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Assay #1: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
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Physical Appearance
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重组人胰岛素样生长因子- 1蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
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Endotoxin
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Less than 0.01 EU/μg of rHuIGF-1 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable for 3 years at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to two weeks at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人胰岛素样生长因子- 1蛋白
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SDS-PAGE
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