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Synonyms
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GH1, GH, GHN, GH-N, hGH-N,Pituitary Growth Hormone, Growth Hormone 1, Somatotropin.
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Species
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Human
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Accession
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P01241
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GeneID
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2688
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Source
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重组人生长**蛋白Escherichia coli.
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Molecular Weight
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Approximately 22 kDa, a single non-glycosylated polypeptide chain containing 191 amino acids
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Quantity
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20µg/100µg/1000µg
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AA Sequence
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FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人生长**蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution with 20mM PB, pH 7.0, with 3% Mannitol.
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Endotoxin
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Less than 1 EU/µg of rHuGH as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人生长**蛋白
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SDS-PAGE
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Reference
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1. Goffin, V., K.T. Shiverick, P.A. Kelly, et al. 1996. Endocr Rev, 17: 385-410.
2. Tannenbaum, G.S., W. Gurd, and M. Lapointe. 1998. Endocrinology, 139: 3871-5.
3. Welniak, L.A., R. Sun, and W.J. Murphy. 2002. J Leukoc Biol, 71: 381-7.
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Background
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重组人生长**蛋白Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is a peptide hormone that stimulates growth, cell reproduction and regeneration in humans and other animals. Growth hormone is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic cells within the lateral wings of the anterior pituitary gland. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones-GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin and leptin. The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified.
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