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Synonyms
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TNFRSF12A, FGF-inducible 14, CD266
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Species
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Human
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Accession
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Q9NP84
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GeneID
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51330
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Source
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Escherichia coli.
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Molecular Weight
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重组体人TNF-related弱诱导细胞凋亡受体/ TNFRSF12A蛋白Approximately 5.6 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids.
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Quantity
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5µg/25µg/1000µg
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AA Sequence
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EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组体人TNF-related弱诱导细胞凋亡受体/ TNFRSF12A蛋白Fully biologically active when compared to standard. The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg, in the presence of 15 ng/ml of rHuTWEAK.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rHuTWEAK Receptor as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组体人TNF-related弱诱导细胞凋亡受体/ TNFRSF12A蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组体人TNF-related弱诱导细胞凋亡受体/ TNFRSF12A蛋白
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Reference
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1. Gaudineau B, Fougere M, Guaddachi F, et al. 2012. J Cell Sci, 125: 4475-86.
2. Abend JR, Uldrick T, Ziegelbauer JM. 2010. J Virol, 84: 12139-51.
3. Kang S, Yang C, Luo R. 2008. Biochem Biophys Res Commun, 372: 629-33.
4. Wiley SRandWinkles JA. 2003. Cytokine Growth Factor Rev, 14: 241-9.
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Background
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Human TNF-related weak inducer of apoptosis receptor (TWEAKR) also known as Tumor necrosis factor receptor superfamily member 12A precursor (gene name TNFRSF12A) or fibroblast growth factor-inducable 14 kD protein, is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. It is expressed in the spleen, thymus, peripheral blood lymphocytes, colon, and small intestine. Signal transduction by TWEAK receptor can be activated by either the membrane anchored or the soluble TWEAK. In addition, It plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. Human and mouse TWEAK R share 82 % a.a. sequence identity.
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