重组人Interleukin-20蛋白 rHuIL-20 上海康朗生物科技有限公司

如果您对该产品感兴趣的话，可以
产品名称：重组人Interleukin-20蛋白
产品型号：rHuIL-20
产品展商：KALANG
产品文档：无相关文档
发布时间：2018-07-16
在线询价

简单介绍

重组人Interleukin-20蛋白与其它公司提供的重组蛋白不同，rHuIL-20蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人Interleukin-20蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品描述

重组人Interleukin-20蛋白

Synonyms	Cytokine Zcyto10
Species	Human
Accession	Q9NYY1
GeneID	50604
Source	重组人Interleukin-20蛋白Escherichia coli.
Molecular Weight	Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity	2µg/10µg/1000µg
AA Sequence	MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重组人Interleukin-20蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.2, with trehalose.
Endotoxin	Less than 1 EU/µg of rHuIL-20 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组人Interleukin-20蛋白
SDS-PAGE
Reference	重组人Interleukin-20蛋白1. Sabat R. 2010. Cytokine Growth Factor Rev, 21: 315-24. 2. Blumberg H, Conklin D, Xu WF, et al. 2001. Cell, 104: 9-19. 3. Wei CC, Hsu YH, Li HH, et al. 2006. J Biomed Sci, 13: 601-12. 4. Hsu YH, Li HH, Hsieh MY, et al. 2006. Arthritis Rheum, 54: 2722-33.
Background	Human Interleukin-20 (IL-20) is encoded by the IL20 gene located on the chromosome 1 and belongs to the IL-10 family that including IL-10, IL-19, IL-22, IL-24, and IL-26. It is secreted by activated keratinocytes and monocytes, and signals through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 has functions of regulating proliferation and differentiation of keratinocytes during inflammation, and causing cell expansion of multipotential hematopoietic progenitor cells.