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Synonyms
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ARMET
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Species
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Human
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Accession
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P55145
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GeneID
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7873
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Source
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重组人中脑的Astrocyte-Derived神经营养因子蛋白Escherichia coli.
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Molecular Weight
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Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing158 amino acids.
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Quantity
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5µg/25µg/1000µg
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AA Sequence
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LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人中脑的Astrocyte-Derived神经营养因子蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rHuMANF as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人中脑的Astrocyte-Derived神经营养因子蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人中脑的Astrocyte-Derived神经营养因子蛋白
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SDS-PAGE
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Reference
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1. Petrova P, Raibekas A, Pevsner J, et al. 2003. J Mol Neurosci. 20:173-88.
2. Airavaara M, Chiocco MJ, Howard DB, et al. 2010. Exp Neurol. 225:104-13.
3. Lindholm P, Peranen J, Andressoo JO, et al. 2008. Mol Cell Neurosci. 39:356-71.
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Background
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MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Mature human MANF is 99 %, 98 % and 96 % a.a. identical to mature rat, mouse and bovine MANF respectively.
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