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重组人Neuregulin 1αEGF域蛋白

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  • 产品名称:重组人Neuregulin 1αEGF域蛋白
  • 产品型号:rHuNRG1-α
  • 产品展商:KALANG
  • 产品文档:无相关文档
  • 发布时间:2018-08-31
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简单介绍
重组人Neuregulin 1αEGF域蛋白与其它公司提供的重组蛋白不同,rHuNRG1-α蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人Neuregulin 1αEGF域蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述

重组人Neuregulin 1αEGF域蛋白

Species Human
Accession Q02297
GeneID 3084
Source Escherichia coli.
Molecular Weight 重组人Neuregulin 1αEGF域蛋白Approximately 7.4 kDa, a single non-glycosylated polypeptide chain containing 65 amino acids.
Quantity 10µg/50µg/1000µg
AA Sequence SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCQPGF TGARCTENVP MKVQNQEKAE ELYQK
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity 重组人Neuregulin 1αEGF域蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered solution in 20 mM PB, pH 6.0, 150 mM NaCl.
Endotoxin Less than 0.1 EU/μg of rHuNRG1-α as determined by LAL method.
Reconstitution 重组人Neuregulin 1αEGF域蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组人Neuregulin 1αEGF域蛋白
SDS-PAGE

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