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Synonyms
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AMAC-1, PARC, DC-CK1
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Species
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Human
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Accession
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P55774
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GeneID
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6362
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Source
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Escherichia coli.
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Molecular Weight
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重组人类巨噬细胞炎性含有/ CCL18蛋白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人类巨噬细胞炎性含有/ CCL18蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 1.0-10 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuMIP-4/CCL18 as determined by LAL method.
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Reconstitution
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重组人类巨噬细胞炎性含有/ CCL18蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人类巨噬细胞炎性含有/ CCL18蛋白
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SDS-PAGE
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Reference
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1. Hieshima K, Imai T, Baba M, et al. 1997. J Immunol, 159: 1140-9.
2. Chenivesse C, Chang Y, Azzaoui I, et al. 2012. J Immunol, 189: 128-37.
3. Azzaoui I, Yahia SA, Chang Y, et al. 2011. Blood, 118: 3549-58.
4. Adema GJ, Hartgers F, Verstraten R, et al. 1997. Nature, 387: 713-7.
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Background
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Human CCL18 is encoded by the CCL18 gene located on the chromosome 17. As also named MIP-4, it shares 61 % sequence identity to human MIP-1α. CCL18 is mainly expressed by lung and some lymphoid tissues like lymph nodes express CCL18 at low level. It is chemotactic for both activated (CD3+) T cells and nonactivated (CD14-) lymphocytes, but not for monocytes or granulocytes. Involved in B-cell migration into B-cell follicles in lymph nodes. CCL18 plays a role in both humoral and cell-mediated immunity responses.
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