|
Synonyms
|
LARC, Beta-chemokine Exodus-1, Liver and Activation-regulated Chemokine
|
|
Species
|
Human
|
|
Accession
|
P78556
|
|
GeneID
|
6364
|
|
Source
|
重组人类巨噬细胞炎性蛋白质3α/ CCL20蛋白Escherichia coli.
|
|
Molecular Weight
|
Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
|
|
Quantity
|
5µg/20µg/1000µg
|
|
AA Sequence
|
ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM
|
|
Purity
|
> 97 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重组人类巨噬细胞炎性蛋白质3α/ CCL20蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50 ng/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
|
|
Endotoxin
|
Less than 1 EU/μg of rHuMIP-3α/CCL20 as determined by LAL method.
|
|
Reconstitution
|
重组人类巨噬细胞炎性蛋白质3α/ CCL20蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组人类巨噬细胞炎性蛋白质3α/ CCL20蛋白
|
|
|
SDS-PAGE
|
|
|
Reference
|
1. Chavan R, Marfatia KA, An IC, et al. 2006. J Virol, 80: 7676-87.
2. Hosokawa Y, Hosokawa I, Ozaki K, et al. 2005. Clin Exp Immunol, 142: 285-91.
3. Hirota K, Yoshitomi H, Hashimoto M, et al. 2007. J Exp Med, 204: 2803-12.
4. Francis JN, Sabroe I, Lloyd CM, et al. 2008. Clin Exp Immunol, 152: 440-7.
|
|
Background
|
CCL20, also named LARC (Liver and Activation-regulated Chemokine), is encoded by CCL20 located on chromosome 2 in humans. The expression can be induced by LPS and some inflammatory cytokines, and it is at a high level in lymph nodes, PBL, fatal liver, fatal lung, appendix and so on. CCL20 has functions of chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. CCL20 elicits it effects by binding with CCR6 receptor.
|
