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Synonyms
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CKB-8, MIP-3, MIPIF-1
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Species
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Human
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Accession
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P55773
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GeneID
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6368
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Source
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Escherichia coli.
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Molecular Weight
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重组人类巨噬细胞炎性蛋白质3 / CCL23蛋白Approximately 11.4 kDa, a single, non-glycosylated polypeptide chain containing 99 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人类巨噬细胞炎性蛋白质3 / CCL23蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuMIP-3/CCL23 as determined by LAL method.
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Reconstitution
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重组人类巨噬细胞炎性蛋白质3 / CCL23蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人类巨噬细胞炎性蛋白质3 / CCL23蛋白
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SDS-PAGE
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Reference
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1. Novak H, Muller A, Harrer N, et al. 2007. J Immunol, 178: 4335-41.
2. Votta BJ, White JR, Dodds RA, et al. 2000. J Cell Physiol, 183: 196-207.
3. Poposki JA, Uzzaman A, Nagarkar DR, et al. 2011. J Allergy Clin Immunol, 128: 73-81 e4.
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Background
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CCL23 is a member of CC chemokine family and is encoded by CCL23 gene located on Chr.17 in humans, where near several other CC chemokines. Highly expressed in ***** lung, liver, skeletal muscle and pancreas, this protein shows strong chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. It elicits the effects by binding to CCR1. CCL23 is reported that it can be cleaved into four forms: CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99).
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