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Synonyms
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TECK, CCL25, SCYA25, Ckb15
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Species
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Human
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Accession
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O15444
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GeneID
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6370
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Source
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重组人胸腺表达趋化因子/ CCL25蛋白Escherichia coli.
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Molecular Weight
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Approximately 14.3 kDa, a single, non-glycosylated polypeptide chain containing 128 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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重组人胸腺表达趋化因子/ CCL25蛋白MQGVFEDCCL AYHYPIGWAV LRRAWTYRIQ EVSGSCNLPA AIFYLPKRHR KVCGNPKSRE VQRAMKLLDA RNKVFAKLHH NTQTFQAGPH AVKKLSSGNS KLSSSKFSNP ISSSKRNVSL LISANSGL
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
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Physical Appearance
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重组人胸腺表达趋化因子/ CCL25蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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Less than 1 EU/µg of rHuTECK/CCL25 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人胸腺表达趋化因子/ CCL25蛋白
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SDS-PAGE
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Reference
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1. Uehara S, Song K, Farber JM, et al. 2002. J Immunol, 168: 134-42.
2. Ericsson A, Svensson M, Arya A, et al. 2004. Eur J Immunol, 34: 2720-9.
3. Svensson MandAgace WW. 2006. Expert Rev Clin Immunol, 2: 759-73.
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Background
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CCL25 is new member of CC family chemokine. It is also called Thymus-expressed chemokine (TECK) because it is restricted produced by thymus and intestine. Especially, the dendritic cells derived from thymus but not bone marrow had identified to be the source of CCL25. By binding with CCR9, it elicits its effects of chemotactic for thymocytes, macrophages, and dendritic cells. Additionally, CCL25 takes part in regulating the development of T-cells.
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