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Synonyms
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CC chemokine IMAC, MIP-4-alpha, TSC-1
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Species
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Human
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Accession
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Q9Y258
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GeneID
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10344
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Source
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重组人嗜酸性粒细胞趋化因子-3 / CCL26蛋白Escherichia coli.
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Molecular Weight
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Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 71 amino acid residues.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人嗜酸性粒细胞趋化因子-3 / CCL26蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CCR3 transfected HEK293 cells is in a concentration range of 0.5-2.0 μg/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rHuEotaxin-3/CCL26 as determined by LAL method.
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Reconstitution
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重组人嗜酸性粒细胞趋化因子-3 / CCL26蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人嗜酸性粒细胞趋化因子-3 / CCL26蛋白
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SDS-PAGE
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Reference
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1. Banwell ME, Tolley NS, Williams TJ, et al. 2002. Cytokine, 17: 317-23.
2. Kagami S, Saeki H, Komine M, et al. 2005. Clin Exp Immunol, 141: 459-66.
3. Stubbs VE, Power C, Patel KD. 2010. Immunology, 130: 74-82.
4. El-Shazly AE, Doloriert HC, Bisig B, et al. 2013. Clin Exp Allergy, 43: 322-31.
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Background
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CCL26 is novel CC family chemokine, also known as TARC (thymus and activation regulated chemokine). It is encoded by CCL26 gene, which is located on Chr.7 in humans, and ubiquitously expressed at low levels in various tissues including heart and ovary. CCL26 elicits its effects activity by binding to the cell surface chemokine receptor CCR3, and chemotactic for eosinophils and basophils.
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