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Synonyms
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Angie, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13
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Species
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Human
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Accession
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O43927
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GeneID
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10563
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Source
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Escherichia coli.
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Molecular Weight
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重组人类BCA-1 / CXCL13蛋白Approximately 10.3 kDa, a single non-glycosylated polypeptide chain containing 87 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/ml.
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Physical Appearance
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重组人类BCA-1 / CXCL13蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuBCA-1/CXCL13 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人类BCA-1 / CXCL13蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人类BCA-1 / CXCL13蛋白
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Reference
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1. Legler DF, Loetscher M, Roos RS, et al. 1998. J Exp Med. 187:655-60.
2. Gunn MD, Ngo VN, Ansel KM, et al. 1998. Nature. 391:799-803.
3. Ansel KM, Harris RB, Cyster JG. 2002. Immunity. 16:67-76.
4. Ansel KM, Ngo VN, Hyman PL, et al. 2000. Nature. 406:309-14.
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Background
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C-X-C motif chemokine 13 encoded by the CXCL13 gene located on human chromosome 4 is constitutively expressed in secondary lymphoid organs. It is also known as B lymphocyte chemoattractant (BLC). As its name suggests, CXCL13 is selectively chemotactic for B cells belonging to both the B-1 and B-2 subsets, and elicits its effects by interacting with chemokine receptor CXCR5. It and its receptor CXCR5 control the organization of B cells within follicles of lymphoid tissues. Recombinant human CXCL13 is a single non-glycosylated polypeptide chain containing 87 amino acids and mature human BCA-1 shares 64 % amino acid sequence similarity with the murine protein and 23–34 % amino acid sequence identity with other known CXC chemokines.
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