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Synonyms
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ATAC, C motif chemokine 1, Cytokine SCM-1, Lymphotaxin, SCM-1-alpha, Small-inducible cytokine C1, XC chemokine ligand 1
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Species
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Human
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Accession
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P47992
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GeneID
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6375
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Source
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重组人**细胞趋化因子/ XCL1蛋白Escherichia coli.
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Molecular Weight
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Approximately 10.2 kDa, a single non-glycosylated polypeptide chain containing 92 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVR SMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人**细胞趋化因子/ XCL1蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuLymphotactin/XCL1 as determined by LAL method.
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Reconstitution
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重组人**细胞趋化因子/ XCL1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人**细胞趋化因子/ XCL1蛋白
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Reference
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1. Kennedy J, Kelner GS, Kleyensteuber S, et al. 1995. J Immunol. 155:203-9.
2. Yoshida T, Imai T, Takagi S, et al. 1996. FEBS Lett. 395:82-8.
3. Yoshida T, Imai T, Kakizaki M, et al. 1998. J Biol Chem. 273:16551-4.
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Background
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Chemokine (C motif) ligand (XCL1), as known as lymphotactin, is the only known member of the C-chemokine family and signals through the receptor XCR1, formally known as GPR5. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces its chemotactic function by binding to a chemokine receptor called XCR1. Recombinant Human XCL1 which is a single non-glycosylated polypeptide chains containing 92 amino acids and it shares approximately 60 % amino acid sequence homology with the murine and rat protein.
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