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- 产品名称：重组人I-309 / CCL1蛋白
- 产品型号：rHuI-309/CCL1
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-13
- 在线询价
简单介绍
重组人I-309 / CCL1蛋白与其它公司提供的重组蛋白不同，rHuI-309/CCL1蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人I-309 / CCL1蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组人I-309 / CCL1蛋白
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Synonyms
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Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
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Species
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Human
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Accession
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P22362
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GeneID
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6346
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Source
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Escherichia coli.
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Molecular Weight
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重组人I-309 / CCL1蛋白Approximately 8.6 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK
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Purity
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>97% by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人I-309 / CCL1蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuI-309/CCL1 as determined by LAL method.
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Reconstitution
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重组人I-309 / CCL1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人I-309 / CCL1蛋白
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SDS-PAGE
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Reference
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1. Miller MD, Krangel MS. 1992. Proc Natl Acad Sci U S A. 89:2950-4.
2. Roos RS, Loetscher M, Legler DF, et al. 1997. J Biol Chem. 272:17251-4.
3. Miller MD, Wilson SD, Dorf ME, et al. 1990. J Immunol. 145:2737-44.
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Background
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Chemokine (C-C motif) ligand 1 (CCL1) belongs to a family inflammatory cytokines and also known as chemokines. It is a small glycoprotein secreted by activated T cells with a molecular weight of approximately 8.5 kDa. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42 % amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines.
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