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Synonyms
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G0/G1 switch regulatory protein 19-1, MIP-1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3,Tonsillar lymphocyte LD78 alpha protein
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Species
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Human
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Accession
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P10147
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GeneID
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6348
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Source
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Escherichia coli.
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Molecular Weight
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重组人巨噬细胞炎症蛋白-1 alpha / CCL3蛋白Approximately 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人巨噬细胞炎症蛋白-1 alpha / CCL3蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuMIP-1α/CCL3 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人巨噬细胞炎症蛋白-1 alpha / CCL3蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人巨噬细胞炎症蛋白-1 alpha / CCL3蛋白
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SDS-PAGE
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Reference
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1. Guan E, Wang J, Norcross MA. 2001. J Biol Chem. 276:12404-9.
2. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5.
3. Nakao M, Nomiyama H, Shimada K. 1990. Mol Cell Biol. 10:3646-58.
4. Czaplewski LG, McKeating J, Craven CJ, et al. 1999. J Biol Chem. 274:16077-84.
5. 1991. Hum Gene Ther. 2:87-96.
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Background
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CCL3, also known as macrophage inflammatory protein-1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1α can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant human MIP-1α is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and it shares approximately 73 %, 74 % and 92 % amino acid sequence homology with the murine, rat and rhesus macaque protein.
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