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Synonyms
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G-26 T-lymphocyte-secreted protein, HC21, LAG-1, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, ACT-2
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Species
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Human
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Accession
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P13236
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GeneID
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6351
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Source
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重组人巨噬细胞炎症蛋白-1 beta / CCL4蛋白Escherichia coli.
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Molecular Weight
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Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人巨噬细胞炎症蛋白-1 beta / CCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuMIP-1β/CCL4 as determined by LAL method.
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Reconstitution
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重组人巨噬细胞炎症蛋白-1 beta / CCL4蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles.
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重组人巨噬细胞炎症蛋白-1 beta / CCL4蛋白
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Reference
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1. Irving SG, Zipfel PF, Balke J, et al. 1990. Nucleic Acids Res. 18:3261-70.
2. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5.
3. Garlisi CG, Xiao H, Tian F, et al. 1999. Eur J Immunol. 29:3210-5.
4. Guan E, Wang J, Roderiquez G, et al. 2002. J Biol Chem. 277:32348-52.
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Background
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CCL4 encoded by the CCL4 gene, also known as Macrophage Inflammatory Protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. It is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Specifically, MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant human CCL4 contains 69 amino acids and it shares 77 % and 80 % a.a. sequence identity with murine and rat CCL4, respectively. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells.
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