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Synonyms
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SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES
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Species
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Human
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Accession
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P13501
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GeneID
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6352
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Source
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重组人调节激活正常T细胞快速序列/ CCL5蛋白Escherichia coli.
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Molecular Weight
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Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人调节激活正常T细胞快速序列/ CCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 100 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuRANTES/CCL5 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles.
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重组人调节激活正常T细胞快速序列/ CCL5蛋白
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SDS-PAGE
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Reference
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重组人调节激活正常T细胞快速序列/ CCL5蛋白1. Hedrick JA, Helms A, Vicari A, et al. 1998. Blood, 91: 4242-7.
2. Nomiyama H, Fukuda S, Iio M, et al. 1999. J Interferon Cytokine Res, 19: 227-34.
3. Youn BS, Zhang S, Broxmeyer HE, et al. 1998. Biochem Biophys Res Commun, 247: 217-22.
4. Kim IS, Jang SW, Sung HJ, et al. 2005. FEBS Lett, 579: 6044-8.
5. Francica G, Petrolati A, Di Stasio E, et al. 2012. Acta Radiol, 53: 394-400.
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Background
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Chemokine (C-C motif) ligand 5 (CCL5) also known as RANTES is classified as a chemotactic cytokine or chemokine. It is encoded by the CCL5 gene in humans. CCL5 is chemotactic for T cells, eosinophils, and basophils, and plays an active role in recruiting leukocytes into inflammatory sites which has been reported to be produced by renal tubular epithelium, synovial fibroblasts and selected tumor cells. It also induces the proliferation and activation of certain natural-killer cells to form CHAK cells. Recombinant human CCL5 contains 68 amino acids and it shares 84 % a.a. sequence identity with murine and rat CCL5, respectively.
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