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- 产品名称：重组人类单核细胞趋化蛋白-3 / CCL7蛋白
- 产品型号：rHuMCP-3/CCL7
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-08-04
- 在线询价
简单介绍
重组人类单核细胞趋化蛋白-3 / CCL7蛋白与其它公司提供的重组蛋白不同，rHuMCP-3/CCL7蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类单核细胞趋化蛋白-3 / CCL7蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组人类单核细胞趋化蛋白-3 / CCL7蛋白
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Synonyms
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NC28, Small-inducible cytokine A7
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Species
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Human
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Accession
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P80098
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GeneID
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6354
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Source
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Escherichia coli.
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Molecular Weight
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重组人类单核细胞趋化蛋白-3 / CCL7蛋白Approximately 9.0 kDa, a single, non-glycosylated polypeptide chain containing 76 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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重组人类单核细胞趋化蛋白-3 / CCL7蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuMCP-3/CCL7 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人类单核细胞趋化蛋白-3 / CCL7蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles.
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重组人类单核细胞趋化蛋白-3 / CCL7蛋白
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SDS-PAGE
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Reference
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1. Opdenakker G, Fiten P, Nys G, et al. 1994. Genomics. 21:403-8.
2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42.
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Background
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Human CCL7, also known as monocyte chemotactic protein 3 (MCP-3), is belonging to the CC chemokine family. MCP-3 has two homogeneous MCP-1 (CCL2) and MCP-2 (CCL8). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL7 shares 71 % and 58 % a.a. sequence identity with MCP-1 and MCP-2, respectively. CCL7 has chemotactic function for monocytes and eosinophils, but not for neutrophils. In addition, it can also chemoattract activated NK cells.
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