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Synonyms
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HC14, Small-inducible cytokine A8
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Species
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Human
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Accession
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P80075
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GeneID
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6355
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Source
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Escherichia coli.
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Molecular Weight
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重组人类单核细胞趋化蛋白-2 / CCL8蛋白Approximately 8.9 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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重组人类单核细胞趋化蛋白-2 / CCL8蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuMCP-2/CCL8 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人类单核细胞趋化蛋白-2 / CCL8蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人类单核细胞趋化蛋白-2 / CCL8蛋白
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SDS-PAGE
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Reference
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1. Van Coillie E, Fiten P, Nomiyama H, et al. 1997. Genomics. 40:323-31.
2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42.
3. Proost P, Wuyts A, Van Damme J. 1996. J Leukoc Biol. 59:67-74.
4. Gong W, Howard OM, Turpin JA, et al. 1998. J Biol Chem. 273:4289-92.
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Background
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Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells.
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