- 如果您对该产品感兴趣的话，可以
- 产品名称：重组人类单核细胞趋化蛋白-4 / CCL13蛋白
- 产品型号：rHuMCP-4/CCL13
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-16
- 在线询价
简单介绍
重组人类单核细胞趋化蛋白-4 / CCL13蛋白与其它公司提供的重组蛋白不同，rHuMCP-4/CCL13蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类单核细胞趋化蛋白-4 / CCL13蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组人类单核细胞趋化蛋白-4 / CCL13蛋白
|
Synonyms
|
CK-beta-10, NCC-1, Small-inducible cytokine A13
|
|
Species
|
Human
|
|
Accession
|
Q99616
|
|
GeneID
|
6357
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重组人类单核细胞趋化蛋白-4 / CCL13蛋白Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 75 amino acids.
|
|
Quantity
|
5µg/20µg/1000µg
|
|
AA Sequence
|
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT
|
|
Purity
|
> 96 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重组人类单核细胞趋化蛋白-4 / CCL13蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
|
|
Endotoxin
|
Less than 1 EU/μg of rHuMCP-4/CCL13 as determined by LAL method.
|
|
Reconstitution
|
重组人类单核细胞趋化蛋白-4 / CCL13蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组人类单核细胞趋化蛋白-4 / CCL13蛋白
|
|
|
Reference
|
1. Uguccioni M, Loetscher P, Forssmann U, et al. 1996. J Exp Med. 183:2379-84.
2. Berkhout TA, Sarau HM, Moores K, et al. 1997. J Biol Chem. 272:16404-13.
3. Garcia-Zepeda EA, Combadiere C, Rothenberg ME, et al. 1996. J Immunol. 157:5613-26.
|
|
Background
|
Human CCL13 is belonging to the CC chemokine family and is encoded by the gene CCL13 in humans. CCL13 (MCP-4) shares 56-61 % sequence identity with MCP-1 (CCL2) and MCP-3 (CCL7) and is 60 % identical to Eotaxin (CCL11). CCL13 was a potent chemoattractant for monocytes and eosinophils and stimulated histamine release from basophils. CCL13 can induce a calcium flux in HEK-293 cells transfected with the receptor CCR2B and CCR3. That shows the function receptors of CCL3 are CCR2B and CCR3.
|
