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Synonyms
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HCC-1/HCC-3, NCC-2, Small-inducible Cytokine A14
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Species
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Human
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Accession
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Q16627
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GeneID
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6358
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Source
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Escherichia coli.
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Molecular Weight
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重组人类血液滤液CC趋化因子-1 / CCL14蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人类血液滤液CC趋化因子-1 / CCL14蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuHCC-1/CCL14 as determined by LAL method.
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Reconstitution
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重组人类血液滤液CC趋化因子-1 / CCL14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8°C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人类血液滤液CC趋化因子-1 / CCL14蛋白
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Reference
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1. Schulz-Knappe P, Magert HJ, Dewald B, et al. 1996. J Exp Med. 183:295-9.
2. Detheux M, Standker L, Vakili J, et al. 2000. J Exp Med. 192:1501-8.
3. Pardigol A, Forssmann U, Zucht HD, et al. 1998. Proc Natl Acad Sci U S A. 95:6308-13.
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Background
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Human CCL14 is belonging to the CC chemokine family. It has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. CCL14 is expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations in plasma.
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