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Synonyms
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C-C Motif Chemokine 15, LKN-1, Mrp-2b, NCC-3, Small-inducible Cytokine A15
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Species
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Human
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Accession
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Q16663
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GeneID
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6359
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Source
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重组人巨噬细胞炎症蛋白-5 / CCL15蛋白Escherichia coli.
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Molecular Weight
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Approximately 10.2 kDa, a single non-glycosylated polypeptide chain containing 92 amino acids.
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Quantity
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5µg/25µg/1000µg
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AA Sequence
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QFINDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人巨噬细胞炎症蛋白-5 / CCL15蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuMIP-5/CCL15 as determined by LAL method.
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Reconstitution
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重组人巨噬细胞炎症蛋白-5 / CCL15蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人巨噬细胞炎症蛋白-5 / CCL15蛋白
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SDS-PAGE
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Reference
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1. Youn BS, Zhang SM, Broxmeyer HE, et al. 1998. Blood. 91:3118-26.
2. Berahovich RD, Miao Z, Wang Y, et al. 2005. J Immunol. 174:7341-51.
3. Pardigol A, Forssmann U, Zucht HD, et al. 1998. Proc Natl Acad Sci U S A. 95:6308-13.
4. Youn BS, Zhang SM, Lee EK, et al. 1997. J Immunol. 159:5201-5.
5. Coulin F, Power CA, Alouani S, et al. 1997. Eur J Biochem. 248:507-15.
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Background
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Human CCL15 is belonging to the CC chemokine family and shares 35 % amino acid homology with human HCC1 (CCL14). CCL15 is most abundant in heart, skeletal muscle and adrenal gland, and low expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. CCL15 has several cleaved chains. All of them are more potent chemoattractants than CCL15.
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