|
Synonyms
|
BMP-2B
|
|
Species
|
Human
|
|
Accession
|
P12644
|
|
GeneID
|
652
|
|
Source
|
重组人骨形态发生蛋白4蛋白Escherichia coli.
|
|
Molecular Weight
|
Approximately 13.3 kDa, a monomeric, non-glycosylated polypeptide chain containing 117 amino acids.
|
|
Quantity
|
2µg/10µg/1000µg
|
|
AA Sequence
|
重组人骨形态发生蛋白4蛋白MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR
|
|
Purity
|
> 95 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Data is not available.
|
|
Physical Appearance
|
重组人骨形态发生蛋白4蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 µm filtered concentrated solution in 50 mM Na2CO3, 5 mM DTT, pH 11.0.
|
|
Endotoxin
|
Less than 1 EU/µg of rHuBMP-4 as determined by LAL method.
|
|
Applications
|
1. Molecular standard (Western, ELISA) in studying secreted BMP-4;
2. Preparing antibodies for BMP-4 monomer;
3. Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组人骨形态发生蛋白4蛋白
|
|
|
SDS-PAGE
|
|
|
Reference
|
1. Yamashita H, Murayama C, Takasugi R, et al. 2011. Mol Cell Biochem, 348: 183-90.
2. Yates KE, Troulis MJ, Kaban LB, et al. 2002. Int J Oral Maxillofac Surg, 31: 173-8.
3. Bessa PC, Cerqueira MT, Rada T, et al. 2009. Protein Expr Purif, 63: 89-94.
4. Kawakami T, Kumasaka M, Kato M, et al. 2011. J Dermatol Sci, 63: 66-9.
|
|
Background
|
Bone Morphogenetic Protein 4 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-4 is expressed in the lung and lower levels seen in the kidney. It also presents in normal and neoplastic prostate tissues, and prostate cancer cell lines. It regulates the formation of teeth, limbs and bone from mesoderm. Furthermore it also plays a role in fracture repair. BMP-4 signals through tetrameric complexes composed of type I and type II receptors and regulates it function by interaction with multiple proteins and glycosaminoglycans. The human BMP-4 shares 98 % sequence indentity with mouse BMP-4. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva.
|
