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Synonyms
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OP-1
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Species
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Human
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Accession
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P18075
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GeneID
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655
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Source
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重组人骨形态发生蛋白7蛋白Escherichia coli.
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Molecular Weight
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Approximately 15.7 kDa, a monomeric, non-glycosylated polypeptide chain containing 139 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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重组人骨形态发生蛋白7蛋白STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDL GWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP KPCCAPTQLN AISVLYFDDS SNVILKKYRN MVVRACGCH
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Data is not available.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.
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Endotoxin
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Less than 1 EU/μg of rHuBMP-7 as determined by LAL method.
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Applications
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1. Molecular standard (Western, ELISA) in studying secreted BMP-7;
2. Preparing antibodies for BMP-7 monomer;
3. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE.
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Reconstitution
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重组人骨形态发生蛋白7蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人骨形态发生蛋白7蛋白
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SDS-PAGE
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Reference
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1. Zeisberg M, Hanai J, Sugimoto H, et al. 2003. Nat Med, 9: 964-8.
2. Phillips FM, Turner AS, Seim HB, 3rd, et al. 2006. Spine J, 6: 500-6.
3. Veerasamy M, Nguyen TQ, Motazed R, et al. 2009. Am J Physiol Renal Physiol, 297: F1238-48.
4. Pauly S, Klatte F, Strobel C, et al. 2012. J Shoulder Elbow Surg, 21: 464-73.
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Background
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Bone Morphogenetic Protein 7 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-7 is mainly expressed in kidney and bladder. It is also present in developing eyes, brain and ear during embryogenesis. BMP-7 also named osteogenic protein-1 (OP-1) is a potent osteoinductive cytokine and plays role in osteoblast differentiation, SMAD1 production and renal development and repair. Human BMP-7 is synthesized with a signal sequence (29 a.a.), a propeptide (263 a.a.), and a growth factor domain (139 a.a.). The growth factor domain of human BMP-7 shares 98 % a.a. sequence identity with mouse and rat BMP-7.
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