|
Synonyms
|
MIP2-alpha
|
|
Species
|
Human
|
|
Accession
|
P19875
|
|
GeneID
|
2920
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重组人类生长调节蛋白-β/ CXCL2蛋白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.
|
|
Quantity
|
2µg/10µg/1000µg
|
|
AA Sequence
|
APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN
|
|
Purity
|
> 97 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重组人类生长调节蛋白-β/ CXCL2蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
|
|
Endotoxin
|
Less than 1 EU/µg of rHuGRO-β/CXCL2 as determined by LAL method.
|
|
Reconstitution
|
重组人类生长调节蛋白-β/ CXCL2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组人类生长调节蛋白-β/ CXCL2蛋白
|
|
|
Reference
|
1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.
2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.
3. Wolpe SD, Sherry B, Juers D, et al. 1989. Proc Natl Acad Sci U S A. 86:612-6.
4. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9.
5. Pelus LM, Fukuda S. 2006. Exp Hematol. 34:1010-20.
|
|
Background
|
CXCL2, also named GRO-β, is belonging to the CXC chemokine family. It is encoded by the gene CXCL2 in humans. CXCL2/GRO-β shares 90 % amino acid sequence with CXCL1/GRO-α. All three human GROs (GRO-α, GRO-β, GRO-γ) are members of the intercrine alpha (chemokine C-X-C) subfamily of chemokine. This chemokine is secreted by monocytes and macrophages. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. Similar to other GRO proteins, CXCL2 is potent neutrophil attractants and activators. In addition, it is also active toward basophils.
|
