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Synonyms
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GRO-gamma, MIP2-beta
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Species
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Human
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Accession
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P19876
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GeneID
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2921
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Source
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Escherichia coli.
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Molecular Weight
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重组人类生长调节蛋白-γ/ CXCL3蛋白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人类生长调节蛋白-γ/ CXCL3蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
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Endotoxin
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Less than 1 EU/µg of rHuGRO-γ/CXCL3 as determined by LAL method.
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Reconstitution
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重组人类生长调节蛋白-γ/ CXCL3蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人类生长调节蛋白-γ/ CXCL3蛋白
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SDS-PAGE
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Reference
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1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.
2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.
3. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84.
4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.
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Background
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CXCL3, also named GRO-γ, is belonging to the CXC chemokine family and encoded by the gene CXCL3. CXCL3/GRO-γ shares 86 % amino acid sequence with CXCL1/GRO-α. All three human GROs (GRO-α, GRO-β, GRO-γ) are members of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is secreted by monocytes and macrophages. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. CXCL3 plays a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three GROs can bind with high affinity to the IL-8 receptor type B.
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