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- 产品名称：重组人血小板因子-4 / CXCL4蛋白
- 产品型号：rHuPF-4/CXCL4
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-09-19
- 在线询价
简单介绍
重组人血小板因子-4 / CXCL4蛋白与其它公司提供的重组蛋白不同，rHuPF-4/CXCL4蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人血小板因子-4 / CXCL4蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组人血小板因子-4 / CXCL4蛋白
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Synonyms
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Iroplact, Oncostatin-A
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Species
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Human
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Accession
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P02776
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GeneID
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5196
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Source
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Escherichia coli.
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Molecular Weight
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重组人血小板因子-4 / CXCL4蛋白Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human fibroblasts is in a concentration of 1.0-10 ng/ml.
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Physical Appearance
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重组人血小板因子-4 / CXCL4蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rHuPF-4/CXCL4 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人血小板因子-4 / CXCL4蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人血小板因子-4 / CXCL4蛋白
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Reference
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1. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
2. Eisman R, Surrey S, Ramachandran B, et al. 1990. Blood. 76:336-44.
3. Lasagni L, Francalanci M, Annunziato F, et al. 2003. J Exp Med. 197:1537-49.
4. Warkentin TE. 2007. N Engl J Med. 356:891-3.
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Background
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Platelet Factor-4 (PF-4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The gene for human CXCL4 is located on human chromosome 4. This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin. It interacts with a splice variant of the chemokine receptor CXCR3 besides be chemotactic for neutrophils, fibroblasts and monocytes. Recombinant human CXCL4 contains 70 amino acids binds with high affinity to heparin. Specifically, Human and mouse CXCL4 share about a 60 % identity.
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