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Synonyms
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ENA-78 (5-78), CXCL5 (5-78), Neutrophil Activating Peptide ENA-78, Small-inducible cytokine B5
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Species
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Human
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Accession
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P42830
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GeneID
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6374
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Source
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Escherichia coli.
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Molecular Weight
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重组人上皮中性粒细胞活化肽-78,5-78 a.a./CXCL5蛋白Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人上皮中性粒细胞活化肽-78,5-78 a.a./CXCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 5.0-10 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuENA-78, 5-78a.a./CXCL5 as determined by LAL method.
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Reconstitution
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重组人上皮中性粒细胞活化肽-78,5-78 a.a./CXCL5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人上皮中性粒细胞活化肽-78,5-78 a.a./CXCL5蛋白
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Reference
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1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82.
2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
3. Persson T, Monsef N, Andersson P, et al. 2003. Clin Exp Allergy. 33:531-7.
4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.
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Background
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CXCL5 is a small cytokine also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). The gene for human CXCL5 is encoded by four exons which belong to CXCL5 gene located on human chromosome 4. The protein is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. In vitro, ENA-78 (8-78) and ENA-78 (9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. They are produced by proteolytic cleavage after secretion from peripheral blood monocytes. Recombinant human CXCL5 (5 - 78 a.a.) contains 74 amino acids and it is a single non-glycosylated polypeptide chain. Human CXCL5 shares 57 % amino acid sequence identity with mouse and rat CXCL5.
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