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Synonyms
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CXCL5, Small-inducible cytokine B5
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Species
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Human
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Accession
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P42830
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GeneID
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6374
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Source
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重组人上皮中性粒细胞激活肽-78,8-78a.a./CXCL5蛋白Escherichia coli.
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Molecular Weight
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Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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LRELRCVCLQ TTQGVHPKMI SNLQVFAIGP QCSKVEVVAS LKNGKEICLD PEAPFLKKVI QKILDGGNKE N
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人上皮中性粒细胞激活肽-78,8-78a.a./CXCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10.0-100.0 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rHuENA-78, 8 - 78 a.a./CXCL5 as determined by LAL method.
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Reconstitution
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重组人上皮中性粒细胞激活肽-78,8-78a.a./CXCL5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人上皮中性粒细胞激活肽-78,8-78a.a./CXCL5蛋白
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Reference
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1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82.
2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
3. Persson T, Monsef N, Andersson P, et al. 2003. Clin Exp Allergy. 33:531-7.
4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.
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Background
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CXCL5 is a member of the CXC chemokine family and also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. In vitro, ENA-78 (8-78) and ENA-78 (9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. They are produced by proteolytic cleavage after secretion from peripheral blood monocytes. Recombinant human CXCL5 (8-78 a.a.) contains 71 amino acids which is a single non-glycosylated polypeptide chain. Human CXCL5 shares 57 % amino acid sequence identity with mouse and rat CXCL5.
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