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- 产品名称：干扰素-γ/ CXCL9诱导的重组人单核因子蛋白
- 产品型号：rHuMIG/CXCL9
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2016-12-20
- 在线询价
简单介绍
干扰素-γ/ CXCL9诱导的重组人单核因子蛋白与其它公司提供的重组蛋白不同，rHuMIG/CXCL9蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.干扰素-γ/ CXCL9诱导的重组人单核因子蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
干扰素-γ/ CXCL9诱导的重组人单核因子蛋白
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Synonyms
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Monokine-like Protein
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Species
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Human
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Accession
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Q07325
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GeneID
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4283
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Source
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Escherichia coli.
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Molecular Weight
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干扰素-γ/ CXCL9诱导的重组人单核因子蛋白Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 103 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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TPVVRKGRCS CISTNQGTIH LQSLKDLKQF APSPSCEKIE IIATLKNGVQ TCLNPDSADV KELIKKWEKQ VSQKKKQKNG KKHQKKKVLK VRKSQRSRQK KTT
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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干扰素-γ/ CXCL9诱导的重组人单核因子蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuMIG/CXCL9 as determined by LAL method.
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Reconstitution
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干扰素-γ/ CXCL9诱导的重组人单核因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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干扰素-γ/ CXCL9诱导的重组人单核因子蛋白
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SDS-PAGE
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Reference
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1. Lee HH, Farber JM. 1996. Cytogenet Cell Genet. 74:255-8.
2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
3. Tensen CP, Flier J, Van Der Raaij-Helmer EM, et al. 1999. J Invest Dermatol. 112:716-22.
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Background
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CXCL9 is a T-cell chemoattractant induced by IFN-γ belonging to the CXC chemokine family and it is also known as Monokine induced by gamma interferon (MIG). CXCL9 is closely related to two other CXC chemokines called CXCL10 and CXCL11 and they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. CXCL9 is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Recombinant human CXCL9 contains 103 amino acids which is a single non-glycosylated polypeptide chain. The human CXCL9 shares 75 % and 67 % a.a. sequence identity with mouse and rat CXCL9.
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