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Synonyms
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Gamma-IP10, Small-inducible Cytokine B10
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Species
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Human
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Accession
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P02778
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GeneID
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3627
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Source
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Escherichia coli.
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Molecular Weight
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干扰素-γ/ CXCL9诱导的重组人单核因子蛋白Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.
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Quantity
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5µg/25µg/1000µg
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AA Sequence
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VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK EMSKRSP
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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干扰素-γ/ CXCL9诱导的重组人单核因子蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
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Endotoxin
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Less than 1 EU/µg of rHuIP-10/CXCL10 as determined by LAL method.
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Reconstitution
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干扰素-γ/ CXCL9诱导的重组人单核因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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干扰素-γ/ CXCL9诱导的重组人单核因子蛋白
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SDS-PAGE
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Reference
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1. Luster AD, Unkeless JC, Ravetch JV. 1985. Nature. 315:672-6.
2. Luster AD, Jhanwar SC, Chaganti RS, et al. 1987. Proc Natl Acad Sci U S A. 84:2868-71.
3. Booth V, Keizer DW, Kamphuis MB, et al. 2002. Biochemistry. 41:10418-25.
4. Dufour JH, Dziejman M, Liu MT, et al. 2002. J Immunol. 168:3195-204.
5. Angiolillo AL, Sgadari C, Taub DD, et al. 1995. J Exp Med. 182:155-62.
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Background
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CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene. CXCL10 was originally identified in monocytes, endothelial cells and fibroblasts as a responsor to IFN-γ. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. The mouse homologue of human IP-10, Crg-2, has been cloned and shown to share approximately 67 % amino acid sequence identity with human IP-10.
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