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Synonyms
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Beta-R1, H174, IP-9, Small-inducible Cytokine B11
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Species
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Human
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Accession
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O14625
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GeneID
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6373
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Source
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Escherichia coli.
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Molecular Weight
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重组人I-TAC / CXCL11蛋白Approximately 8.3 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQ ARLIIKKVER KNF
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人I-TAC / CXCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human IL-2 activated human T-lymphocytes is in a concentration range of 0.1-10 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
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Endotoxin
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Less than 1 EU/µg of rHuI-TAC/CXCL11 as determined by LAL method.
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Reconstitution
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重组人I-TAC / CXCL11蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人I-TAC / CXCL11蛋白
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SDS-PAGE
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Reference
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1. Tensen CP, Flier J, Rampersad SS, et al. 1999. Biochim Biophys Acta. 1446:167-72.
2. Cole KE, Strick CA, Paradis TJ, et al. 1998. J Exp Med. 187:2009-21.
3. Rani MR, Foster GR, Leung S, et al. 1996. J Biol Chem. 271:22878-84.
4. Tensen CP, Flier J, Van Der Raaij-Helmer EM, et al. 1999. J Invest Dermatol. 112:716-22.
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Background
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CXCL11 also known as I-TAC is belonging to the CXC chemokine family and shares 36 % and 37 % amino acid sequence homology with IP-10 and MIG, respectively. It is highly expressed in peripheral blood leukocytes, pancreas and liver. Expression of CXCL11 is strongly induced by IFN-γ and IFN-β, and weakly induced by IFN-α. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3, which with a higher affinity than do the other chemokines for this receptor, CXCL9 and CXCL10. Similar to CXCL10, CXCL11 has been shown to be a chemoattractant for IL-2-activated T-lymphocytes, but not for isolated T-cells, neutrophils or monocytes.
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