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- 产品名称：重组人干扰素-α2a蛋白
- 产品型号：rHuIFN-α2a
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-09-15
- 在线询价
简单介绍
重组人干扰素-α2a蛋白与其它公司提供的重组蛋白不同，rHuIFN-α2a蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人干扰素-α2a蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组人干扰素-α2a蛋白
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Species
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Human
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Accession
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P01563
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GeneID
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3440
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Source
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Yeast
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Molecular Weight
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Approximately 19.2 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.
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Quantity
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20µg/100µg/1000µg
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AA Sequence
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重组人干扰素-α2a蛋白CDLPQTHSLG SRRTLMLLAQ MRKISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 × 108 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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重组人干扰素-α2a蛋白Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rHuIFN-α2a as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人干扰素-α2a蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人干扰素-α2a蛋白
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SDS-PAGE
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Reference
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1. Tarhini AA, Gogas H, Kirkwood JM. 2012. J Immunol, 189: 3789-93.
2. Zarogoulidis P, Kioumis I, Papanas N, et al. 2012. J Chemother, 24: 173-7.
3. Tohyama M, Yang L, Hanakawa Y, et al. 2012. J Invest Dermatol, 132: 1933-5.
4. Corssmit EP, Heijligenberg R, Hack CE, et al. 1997. Clin Exp Immunol, 107: 359-63.
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Background
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IFN-αs are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-α family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
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