重组人成纤维细胞生长因子19蛋白 rHuFGF-19 上海康朗生物科技有限公司

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产品名称：重组人成纤维细胞生长因子19蛋白
产品型号：rHuFGF-19
产品展商：KALANG
产品文档：无相关文档
发布时间：2018-07-24
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简单介绍

重组人成纤维细胞生长因子19蛋白与其它公司提供的重组蛋白不同，rHuFGF-19蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人成纤维细胞生长因子19蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品描述

重组人成纤维细胞生长因子19蛋白

Species	Human
Accession	O95750
GeneID	9965
Source	Escherichia coli.
Molecular Weight	Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids.
Quantity	重组人成纤维细胞生长因子19蛋白5µg/25µg/1000µg
AA Sequence	MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重组人成纤维细胞生长因子19蛋白Assay #1: Fully biologically active when compared to standard. The biological activity is measured by its binding ability in a functional ELISA. Immobilized rHuFGF R4 at 5 µg/ml can bind rHuFGF-19 with a linear range of 3-200 ng/ml. Assay #2: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 10³ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	重组人成纤维细胞生长因子19蛋白Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/µg of rHuFGF-19 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组人成纤维细胞生长因子19蛋白
Reference	1. Ladher RK, Anakwe KU, Gurney AL, et al. 2000. Science. 290:1965-7. 2. Tomlinson E, Fu L, John L, et al. 2002. Endocrinology. 143:1741-7. 3. Smallwood PM, Munoz-Sanjuan I, Tong P, et al. 1996. Proc Natl Acad Sci U S A. 93:9850-7. 4. Fu L, John LM, Adams SH, et al. 2004. Endocrinology. 145:2594-603. 5. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35. 6. Kurosu H, Kuro OM. 2009. Mol Cell Endocrinol. 299:72-8. 7. Lin BC, Wang M, Blackmore C, et al. 2007. J Biol Chem. 282:27277-84. 8. Kharitonenkov A, Dunbar JD, Bina HA, et al. 2008. J Cell Physiol. 215:1-7.
Background	Human FGF-19 is encoded by the FGF19 gene. FGF-19 belongs to the FGF-19 subfamily which has three members FGF-19, 21, 23. FGFs are classically considered to be paracrine factors and are known for their roles in tissue patterning and organogenesis during embryogenesis. By contrast, the FGF-19 subfamily has recently been shown to function in an endocrine manner. Members of this subfamily have poor ability of binding to heparin binding site which is a crucial factor in ligand-receptor complex formation. β-Klotho has been identified as co-factor required for FGF-19, 21, 23 signaling. It can obviously increase ligand-receptor affinity. Unlike most FGFs which bind to and activate more than one FGF receptor, FGF19 is a specific ligand for FGF R4. In FGF-19 transgenic mice, reducing liver triglycerides, increasing fatty acid oxidation, reducing glucose levels and improving insulin sensitivity can be observed.