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Synonyms
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Interferon alpha-II-1
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Species
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Human
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Accession
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P05000
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GeneID
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3467
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 20.0 kDa, containing 172 amino acid residues with two conserved disulfide bonds.
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Quantity
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20µg/100µg/1000µg
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AA Sequence
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重组人干扰素-ω蛋白CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LQQIFSLFHT ERSSAAWNMT LLDQLHTGLH QQLQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human TF-1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.
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Physical Appearance
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重组人干扰素-ω蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rHuIFN-ω as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人干扰素-ω蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人干扰素-ω蛋白
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SDS-PAGE
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Reference
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1. Yang LM, Xue QH, Sun L, et al. 2007. J Interferon Cytokine Res, 27: 119-27.
2. Liu L, Leaman DW, Bixby JA, et al. 1996. Biochim Biophys Acta, 1294: 55-62.
3. Adolf GR. 1990. Virology, 175: 410-7.
4. Benoit P, Maguire D, Plavec I, et al. 1993. J Immunol, 150: 707-16.
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Background
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Interferon-Omega (IFN-ω) coded by IFNW1 gene in human, is a number of the type I interferon family, which includes IFN-α, IFN-β, and IFN-ω. The IFNAR-1/IFNAR-2 receptor complex can help with the signal transduction, followed the antiviral or the antiproliferative actions. IFN-ω is derived from IFN-α/β and share 75 % sequence with IFN-α. It has two intramolecular disulfide bonds which are crucial for activities. Mire-Sluis et al have described bioassays for IFN-α, IFN-β, and IFN-ω that exploit the ability of these factors to inhibit proliferation of TF-1 cells induced by GM-CSF. The bioassays can be used also with Epo and TF-1 cells, or Epo and Epo-transfected UT-7 cells.
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