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- 产品名称：重组人胶质细胞成熟因子beta蛋白
- 产品型号：rHuGMF-β
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-24
- 在线询价
简单介绍
重组人胶质细胞成熟因子beta蛋白与其它公司提供的重组蛋白不同，rHuGMF-β蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人胶质细胞成熟因子beta蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组人胶质细胞成熟因子beta蛋白
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Synonyms
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GMFB
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Species
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Human
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Accession
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P60983
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GeneID
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2764
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Source
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Escherichia coli.
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Molecular Weight
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重组人胶质细胞成熟因子beta蛋白Approximately 16.6 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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SESLVVCDVA EDLVEKLRKF RFRKETNNAA IIMKIDKDKR LVVLDEELEG ISPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPVGCKPEQQ MMYAGSKNKL VQTAELTKVF EIRNTEDLTE EWLREKLGFF H
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Data Not Available.
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Physical Appearance
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重组人胶质细胞成熟因子beta蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rHuGMF-β as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人胶质细胞成熟因子beta蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人胶质细胞成熟因子beta蛋白
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Reference
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1. Gandhi M, Smith BA, Bovellan M, et al. 2010. Curr Biol, 20: 861-7.
2. Roman AandTombarkiewicz B. 2009. Bioelectromagnetics, 30: 21-8.
3. Ohnishi T, Arita N, Hayakawa T, et al. 1993. Biochem Biophys Res Commun, 193: 518-25.
4. Nakano K, Kuwayama H, Kawasaki M, et al. 2010. Cytoskeleton (Hoboken), 67: 373-82.
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Background
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The glia maturation factor beta belongs to the actin-binding proteins ADF family, GMF subfamily. It contains an ADF-H domain, but the research of crystallography and NMR reveals that there are structures different between human and mouse ADF-H domain. GMF-β is involved in the differentiation, maintenance, and regeneration of the nervous system. It also inhibition of proliferation of tumor cells.
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