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Synonyms
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Brain natriuretic peptide 32, Gamma-brain natriuretic peptide, B-type Natriuretic Peptide, GC-B, BNP-32
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Species
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Human
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Accession
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P16860
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GeneID
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4879
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Source
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Escherichia coli.
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Molecular Weight
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重组人脑钠素肽蛋白Approximately 3.5 kDa, a single non-glycosylated polypeptide chain containing 32 amino acids.
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Quantity
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20µg/100µg/1000µg
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AA Sequence
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SPKMVQGSGC FGRKMDRISS SSGLGCKVLR RH
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人脑钠素肽蛋白Data Not Available.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rHuBNP as determined by LAL method.
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Reconstitution
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重组人脑钠素肽蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人脑钠素肽蛋白
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Reference
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1. Matsuo H, Kangawa K, Minamino N. 1988. Tanpakushitsu Kakusan Koso, 33: 2438-50.
2. Uehara Y, Shimizu H, Shimomura Y, et al. 1990. Neuropeptides, 17: 107-10.
3. Hunt PJ, Espiner EA, Nicholls MG, et al. 1997. Peptides, 18: 1475-81.
4. Richards AM, Lainchbury JG, Nicholls MG, et al. 2002. Trends Endocrinol Metab, 13: 151-5.
5. Trojnarska O, Gwizdala A, Katarzynski S, et al. 2010. Int J Cardiol, 139: 241-7.
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Background
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Brain Natriuretic Peptide is encoded by the BNP gene located on the Chr.1 in humans. It is firstly discovered in the porcine brain and given this name, but the protein is mainly expressed in the cardiac ventricles in human body after the excessive stretching of cardiomyocytes. The gene expresses a 134 a.a. sequence which contains a 1-26 a.a. signal peptide and 27-134 a.a. Natriuretic peptides B, and the BNP is the 32 a.a. C-terminus of natriuretic peptides B. The BNP can be cleaved in 16 chains and the rHuBNP is 1-32. BNP acts as a cardiac hormone with a variety of functions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Additionnaly, it plays a key role in cardiovascular homeostasis, helps restore the body's salt and water balance and improves heart function.
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