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Synonyms
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Cytotoxic T-lymphocyte-associated antigen 8, CLTA-8 , IL-17A
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Species
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Human
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Accession
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Q16552
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GeneID
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3605
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Source
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重组人白细胞介素-17蛋白Escherichia coli.
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Molecular Weight
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Approximately 31.0 kDa, a disulfide-linked homodimer of two 132 amino acid polypeptide chains.
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Quantity
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5µg/25µg/1000µg
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AA Sequence
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GITIPRNPGC PNSEDKNFPR TVMVNLNIHN RNTNTNPKRS SDYYNRSTSP WNLHRNEDPE RYPSVIWEAK CRHLGCINAD GNVDYHMNSV PIQQEILVLR REPPHCPNSF RLEKILVSVG CTCVTPIVHH VA
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Purity
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重组人白细胞介素-17蛋白> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 7.5 ng/ml, corresponding to a specific activity of > 1.3 × 105 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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重组人白细胞介素-17蛋白Less than 1 EU/µg of rHuIL-17 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人白细胞介素-17蛋白
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SDS-PAGE
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Reference
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1. Mungall AJ, Palmer SA, Sims SK, et al. 2003. Nature, 425: 805-11.
2. Kolls JKandLinden A. 2004. Immunity, 21: 467-76.
3. Fossiez F, Djossou O, Chomarat P, et al. 1996. J Exp Med, 183: 2593-603.
4. Yao Z, Painter SL, Fanslow WC, et al. 1995. J Immunol, 155: 5483-6.
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Background
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Human Interleukin-17A (IL-17A) is encoded by the IL17A gene located on the chromosome 6 and belongs to the IL-17 family that contains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. They have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, but no sequence similarity to any other known cytokines. Interleukin 17 is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, IL-17 exhibits 63 % amino acid identity with mouse IL-17. High levels of human IL-17 were induced from primary peripheral blood CD4+ T cells upon stimulation and they can induce stromal cells to produce proinflammatory and hematopoietic cytokines.
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