重组人白细胞介素-19蛋白 rHuIL-19 上海康朗生物科技有限公司

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产品名称：重组人白细胞介素-19蛋白
产品型号：rHuIL-19
产品展商：KALANG
产品文档：无相关文档
发布时间：2018-08-14
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简单介绍

重组人白细胞介素-19蛋白与其它公司提供的重组蛋白不同，rHuIL-19蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人白细胞介素-19蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品描述

重组人白细胞介素-19蛋白

Synonyms	Melanoma Differentiation-associated Protein-like Protein, NG.1
Species	Human
Accession	Q9UHD0
GeneID	29949
Source	Escherichia coli.
Molecular Weight	重组人白细胞介素-19蛋白Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity	2µg/10µg/1000µg
AA Sequence	LRRCLISTDM HHIEESFQEI KRAIQAKDTF PNVTILSTLE TLQIIKPLDV CCVTKNLLAF YVDRVFKDHQ EPNPKILRKI SSIANSFLYM QKTLRQCQEQ RQCHCRQEAT NATRVIHDNY DQLEVHAAAI KSLGELDVFL AWINKNHEVM SSA
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重组人白细胞介素-19蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 1.5 ng/ml, corresponding to a specific activity of > 6.7 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	重组人白细胞介素-19蛋白Less than 1 EU/µg of rHuIL-19 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100 mM HAC to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组人白细胞介素-19蛋白
Reference	1. Chang C, Magracheva E, Kozlov S, et al. 2003. J Biol Chem, 278: 3308-13. 2. Gallagher G, Dickensheets H, Eskdale J, et al. 2000. Genes Immun, 1: 442-50. 3. Liao YC, Liang WG, Chen FW, et al. 2002. J Immunol, 169: 4288-97.
Background	Human Interleukin-19 (IL-19) is encoded by the IL19 gene, which is located on the chromosome 1. The protein belongs to the IL-10 family that includes IL-10, IL-20, IL-22, IL-24, and IL-26. As a monomer made of seven amphipathic helices, IL-19 has a helical bundle and shares the same cell surface receptor (IL-20R) with IL-20 and IL-24. It may play some important roles in inflammatory responses because it up-regulates IL-6 and TNF-alpha and induces apoptosis.