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Species
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Human
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Accession
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Q6EBC2
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GeneID
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386653
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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重组人白介素-31蛋白SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5 ng/mL of rHuIL-31 can effectively induce STAT3 activation.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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重组人白介素-31蛋白Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1 EU/µg of rHuIL-31 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人白介素-31蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人白介素-31蛋白
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Reference
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1. Sonkoly E, Muller A, Lauerma AI, et al. 2006. J Allergy Clin Immunol, 117: 411-7.
2. Jawa RS, Chattopadhyay S, Tracy E, et al. 2008. J Interferon Cytokine Res, 28: 207-19.
3. Takaoka A, Arai I, Sugimoto M, et al. 2005. Eur J Pharmacol, 516: 180-1.
4. Ohmatsu H, Sugaya M, Suga H, et al. 2012. Acta Derm Venereol, 92: 282-3
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Background
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Human IL-31 gene is located on Chr.12. It expresses the IL-31 protein at low levels in the type 2 helper T cells, which exits in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. This protein shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. IL-31 signals through IL-31 receptor A and oncostatin M receptor subunits and can activate STAT3 through receptors and maybe involve in skin immunity. It regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Human IL-31 shares 24 % a.a. sequence identity in the mature protein with mouse IL-31.
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