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Synonyms
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IL-1F11, NF-HEV, DVS 27
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Species
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Human
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Accession
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O95760
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GeneID
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90865
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Source
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Escherichia coli.
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Molecular Weight
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重组人白细胞介素-33蛋白Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人白细胞介素-33蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, 150 mM NaCl, 1mM EDTA, pH7.4.
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Endotoxin
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Less than 1 EU/µg of rHuIL-33 as determined by LAL method.
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Reconstitution
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重组人白细胞介素-33蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人白细胞介素-33蛋白
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SDS-PAGE
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Reference
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1. Liew FY, Pitman NI, McInnes IB. 2010. Nat Rev Immunol, 10: 103-10.
2. Miller AM, Xu D, Asquith DL, et al. 2008. J Exp Med, 205: 339-46.
3. Bourgeois E, Van LP, Samson M, et al. 2009. Eur J Immunol, 39: 1046-55.
4. Zhiguang X, Wei C, Steven R, et al. 2010. Immunol Lett, 131: 159-65.
5. Turnquist HR, Zhao Z, Rosborough BR, et al. 2011. J Immunol, 187: 4598-610.
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Background
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IL-33, encoded by IL-33 gene located on the Chr.9 in humans, is a 30 kDa proinflammatory protein belonging to the IL-1 superfamily and it shares less than 20 % a.a. sequence identity with other members. IL-33 secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL1α or IL1β stimulation. It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 mature protein has 52-58 % a.a. sequence identity with mouse and rat IL-33.
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