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Synonyms
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IL-1RN, IRAP
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Species
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Human
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Accession
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P18510
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GeneID
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3557
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Source
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重组人白细胞介素-1受体拮抗剂蛋白蛋白Escherichia coli.
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Molecular Weight
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Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
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Quantity
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20µg/100µg/1000µg
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AA Sequence
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RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人白细胞介素-1受体拮抗剂蛋白蛋白Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg in the presence of 50 pg/ml rHuIL-1α.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rHuIL-1RA as determined by LAL method.
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Reconstitution
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重组人白细胞介素-1受体拮抗剂蛋白蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人白细胞介素-1受体拮抗剂蛋白蛋白
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SDS-PAGE
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Reference
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1. Dinarello, C.A. 1994. FASEB J, 8: 1314-25.
2. Perrier, S., F. Darakhshan, and E. Hajduch. 2006. FEBS Lett, 580: 6289-94.
3. Dinarello, C.A. 1994. FASEB J, 8: 1314-25.
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Background
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Interleukin-1 receptor antagonist (IL-1RA) is a member of the IL-1 family. IL1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL1β. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-RA. The regulated expression of IL1RA in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1RA is markedly enhanced by IL-1, TNF-alpha, or PDGF. The protein shows 26 % amino acid homology to IL1β and 19 % homology to IL1α.
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